Tag
3xHA

Part:BBa_K2984073:Design

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-19)


3x HA-Tag for protein detection, isolation, purification


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was used to make our composite parts compatible for the iGEM registry. This part is not MoClo compatible because it does not have the MoClo overhangs!


Source

Silflow, C. D., LaVoie, M., Tam, L. W., Tousey, S., Sanders, M., Wu, W., . . . Lefebvre, P. A. (2001). The Vfl1 Protein in Chlamydomonas localizes in a rotationally asymmetric pattern at the distal ends of the basal bodies. The Journal of cell biology, 153(1), 63-74. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/11285274

References